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crispr activation plasmid  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology crispr activation plasmid
    Crispr Activation Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+crispr+activation+plasmids/Control+CRISPR+Activation+Plasmid/pm41274424-75-1-5
    Average 94 stars, based on 131 article reviews
    crispr activation plasmid - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    shRNA:

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β -actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β-actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Control:

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β -actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β-actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3
    Article Snippet: The Cell Proliferation ELISA, BrdU (colorimetric) Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). .. The Gal-3 and Control CRISPR Activation Plasmids for mouse cells, UltraCruz ® Transfection Reagent, and Plasmid Transfection Medium were purchased from Santa Cruz Biotechnology, Inc.. .. The pGL4.10[ luc2 ] Vector, pGL4.74[ hRluc /TK] Vector, pmirGLO Dual-Luciferase miRNA Target Expression Vector, FuGENE ® HD Transfection Reagent, and Dual-Glo ® Luciferase Assay System were purchased from Promega (Beijing) Biotech Co., Ltd. (Beijing, China).

    CRISPR:

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β -actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β-actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3
    Article Snippet: The Cell Proliferation ELISA, BrdU (colorimetric) Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). .. The Gal-3 and Control CRISPR Activation Plasmids for mouse cells, UltraCruz ® Transfection Reagent, and Plasmid Transfection Medium were purchased from Santa Cruz Biotechnology, Inc.. .. The pGL4.10[ luc2 ] Vector, pGL4.74[ hRluc /TK] Vector, pmirGLO Dual-Luciferase miRNA Target Expression Vector, FuGENE ® HD Transfection Reagent, and Dual-Glo ® Luciferase Assay System were purchased from Promega (Beijing) Biotech Co., Ltd. (Beijing, China).

    Activation Assay:

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β -actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.
    Article Snippet: Rabbit polyclonal antibodies against GAPDH, β-actin, Thioredoxin 1 (Trx1), Apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK), and its phosphorylated-form (Thr183/Tyr185), p38 mitogen-activated protein kinases (MAPK) and its phosphorylated-form (Thr180/Tyr182), full-length and cleaved poly ADP-ribose polymerase (PARP), and full-length and cleaved caspase 3 were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). .. Horseradish peroxidase (HRP)-conjugated secondary antibody (goat anti-rabbit), human TrxR1 shRNA plasmids, control shRNA plasmids, TrxR1 CRISPR activation plasmids, control CRISPR activation plasmids were obtained from Santa Cruz Biotech. .. Gibco fetal bovine serum (FBS), Iscove's Modified Dulbecco's Medium (IMDM) cultural media, phosphate buffer saline (PBS), and penicillin-streptomycin solution were purchased from Thermo-Fisher Scientific.

    Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3
    Article Snippet: The Cell Proliferation ELISA, BrdU (colorimetric) Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). .. The Gal-3 and Control CRISPR Activation Plasmids for mouse cells, UltraCruz ® Transfection Reagent, and Plasmid Transfection Medium were purchased from Santa Cruz Biotechnology, Inc.. .. The pGL4.10[ luc2 ] Vector, pGL4.74[ hRluc /TK] Vector, pmirGLO Dual-Luciferase miRNA Target Expression Vector, FuGENE ® HD Transfection Reagent, and Dual-Glo ® Luciferase Assay System were purchased from Promega (Beijing) Biotech Co., Ltd. (Beijing, China).

    Transfection:

    Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3
    Article Snippet: The Cell Proliferation ELISA, BrdU (colorimetric) Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). .. The Gal-3 and Control CRISPR Activation Plasmids for mouse cells, UltraCruz ® Transfection Reagent, and Plasmid Transfection Medium were purchased from Santa Cruz Biotechnology, Inc.. .. The pGL4.10[ luc2 ] Vector, pGL4.74[ hRluc /TK] Vector, pmirGLO Dual-Luciferase miRNA Target Expression Vector, FuGENE ® HD Transfection Reagent, and Dual-Glo ® Luciferase Assay System were purchased from Promega (Beijing) Biotech Co., Ltd. (Beijing, China).

    Plasmid Preparation:

    Article Title: Berberine inhibits adipocyte differentiation, proliferation and adiposity through down-regulating galectin-3
    Article Snippet: The Cell Proliferation ELISA, BrdU (colorimetric) Kit was purchased from Roche Applied Science (Indianapolis, IN, USA). .. The Gal-3 and Control CRISPR Activation Plasmids for mouse cells, UltraCruz ® Transfection Reagent, and Plasmid Transfection Medium were purchased from Santa Cruz Biotechnology, Inc.. .. The pGL4.10[ luc2 ] Vector, pGL4.74[ hRluc /TK] Vector, pmirGLO Dual-Luciferase miRNA Target Expression Vector, FuGENE ® HD Transfection Reagent, and Dual-Glo ® Luciferase Assay System were purchased from Promega (Beijing) Biotech Co., Ltd. (Beijing, China).



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